روش ها و پروتکل PCR کمی زمان واقعی

عنوان فارسی:      PCR کمّی زمان واقعی: روش ها و پروتکل ها

عنوان انگلیسی:      Quantitative Real-Time PCR: Methods and Protocols

This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study

• Includes cutting-edge techniques
• Provides step-by-step detail essential for reproducible results
• Contains key implementation advice from the experts

1. A Quarter Century of PCR-Applied Techniques and Their Still-Increasing Fields of Use
2. Parameters for Successful PCR Primer Design
3. MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing
4. Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A) Tailing and 5′ Adaptor Ligation
5. A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PC R
6. Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR)
7. Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR)
8. Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine
9. A General Protocol for Accurate Gene Expression Analysis in Plants
10. Gene Expression Analysis in Bacteria by RT-qPCR
11. Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR
12. Molecular Monitoring of Chronic Myeloid Leukemia
13. Normalization in Human Glioma Tissue
14. qPCR Applications for the Determination of the Biological Age
15. QuantStudio™ 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis
16. Digital PCR and the QuantStudio™ 3D Digital PCR System